CCRES Microalgae Process Design
Join the ranks of hundreds of
Energy Day organisers across Europe for the
2015 EU Sustainable Energy Week!
CCRES Microalgae Process Design
Join the ranks of hundreds of
Energy Day organisers across Europe for the
2015 EU Sustainable Energy Week!
CCRES Microalgae Process Design
#Fucose is an essential hexose deoxy sugar the human body needs to optimally communicate from cell to cell. Simply put, it plays an important role in transmitting information in the brain. Research studies show that this sugar stimulates brain development and can also influence the brain to be able to create long-term memories. This is further supported by studies in which doctors inhibited protein containing fucose; amnesia was the result.
Fucose is found in a number of places in the human body. Its location in the male testes suggests that it may play an important role during reproduction. Also found in the epidermis, it may help in maintaining skin hydration. Beyond these locations, this sugar is found at the articulation between each nerve, in the tubules of the human kidney, and in significant quantities in human breast milk.
It’s important not to confuse this with the similar sounding fructose. While both are sugars that can be commonly found in the body, fructose is a simple monosaccharide sugar found in many foods. For example, you can find a high amount of fructose in baby food, salad dressing, blackberries, tree fruits, honey and even some root vegetables. On the other hand, fucose, as previously stated, can be found in the human body naturally.
Studies also show that fucose may play a role in certain diseases, such as cancer and its infection method. Though research is not yet conclusive, there is promise shown for using fucose to inhibit both breast cancer and leukemia, in addition to tumor growth, in general. Some studies have even gone as far as to conclude that this hexose deoxy sugar seems to be among the most effective sugars at attempting to prevent cancer cells from growing.
Research indicates that even taking in fucose in extremely high amounts does not seem to present any real ill side effects, though recommendations are that the average 150-pound (68.2 kg) human adult can safely handle 34 grams of this sugar on a daily basis. During urination, fucose leaves the body, so people who urinate frequently can experience a deficiency in fucose. People with rheumatoid arthritis also generally are deficient in this kind of sugar. Many people opt to take supplements to ensure they have the right amount in their body. Seaweeds such as kelp, beer yeast, and medicinal mushrooms are also a good alternative to supplements and for people who have difficulty taking pills.
#CCRES #ALGAE TEAM
市場調査レポート: 藻類バイオ燃料技術：世界市場および製品動向(2010年～2015年)Algae Biofuels Technologies – Global Market and Product Trends 2010-2015
Work, Sport, Leisure – in fact all physical activity will generate reactive oxygen species (ROS); the more intense the activity the greater number of free radicals. ROS are shown to have damaging effects on muscle performance and recovery. Published and on-going research, focused on improving endurance and reducing recovery time, are showing dramatic benefits linked to the potent carotenoid – astaxanthin. These findings are bringing astaxanthin to the forefront as a dietary supplement for professional athletes and physically active people.
In a randomized, double-blind, placebo controlled study on healthy men supplemented with 4 mg astaxanthin per day for up to 6 months at Karolinska Institute, Sweden, standardized exercise tests demonstrated that the average number of knee bends performed increased only in the astaxanthin treated group at 3 months, and by the 6 month significant improvements were observed (Figure 1) (Malmsten & Lignell, 2008).
In another study, Aoi et al., (2008) demonstrated that astaxanthin may modify muscle metabolism by its antioxidant property and result in improved muscle performance and weight loss benefits. After 4 weeks the mice running time to exhaustion had significantly improved by up to 20 % , (2002) of Juntendo University, Japan, demonstrated by using 1200 meter track athletes, that a daily dose of 6 mg per day for 4 weeks resulted in their bodies accumulating lower levels of lactic acid (Figure 3). Ikeuchi et al., (2006) also reported the same findings and furthermore, astaxanthin efficacy had a dose-dependent response (Figure 4).
In a double blind controlled placebo study, healthy women (n= 32; age-23-60) who ingested 12 mg of astaxanthin for 6 weeks significantly reduced their body fat (4%) when conducting routine walking exercise, compared to a placebo group. In addition, while control group increased their lactic acid by 31% compared to the astaxanthin group – only 13%
The mechanism behind muscle endurance is based on several findings. Generally, astaxanthin protected the skeletal muscle from the increased damage of oxidative stress generated by physical activity. Furthermore, astaxanthin increased the metabolism of lipids as the main source of energy production by protecting the carnitine palmitoyltransferase I (CPT I) involved in fatty acid transport into mitochondria. Aoi et al., (2003) of Kyoto Prefecture University used mice models that may partially explain the efficacy of astaxanthin; they compared control, exercise placebo, and astaxanthin treated exercise groups after intense physical activity. 4-hydroxy-2-nonenal-modified-protein (4-HNE) stain analyses of the calf (gastrocnemius) muscles revealed significantly lower peroxidation damage (Figure 5).
Other biochemical markers for oxidative damage and inflammation such as DNA, (2003) also explained that astaxanthin directly modulates inflammation caused by the release of the pro-inflammatory cytokines and mediators. In vivo and in vitro tests demonstrate that astaxanthin inhibits the IκB Kinase (IKK) dependant activation of the Nuclear Factor-kB (NF-κB) pathway, a key step in the production of pro-inflammatory cytokines and mediators. Aoi et al., 2008 also demonstrated increased lipid metabolism compared to carbohydrate as the main source of energy during strenuous activity (Figure 6). Furthermore, analysis of the mitochondrial lipid transport enzyme known as carnitine palmitoyltransferase I (CPT I) revealed increased fat localization (Figure 7) and reduction of oxidative damage in the presence of astaxanthin (Figure 8). CPT I is important because it regulates fatty acyl-CoA entry into the mitochondria in the oxidation of fatty acids in muscle. Exercise-induced ROS may partly limit utilization of fatty acid via diminishing CPT I activity.
Strenuous physical activity generates high levels of ROS which affect muscle performance and metabolism of lipids. New research shows that astaxanthin can modify muscle metabolism via its antioxidant effect, resulting in the improvement of muscle function during exercise. Therefore, astaxanthin is expected to be useful for physically active people as well as athletes.
Croatian Center of Renewable Energy Sources (CCRES)
High blood pressure, high levels of triglycerides, oxidation of Low Density Lipoprotein (LDL) cholesterol and lowering levels of High Density Lipoprotein (HDL) cholesterol are the primary cause that leads to oxidative stress and chronic inflammation in the vessels. This condition emerges at early age and gradually compromises vascular integrity leading to atherosclerosis at a later stage of a person lifespan. Atherosclerosis is a cardiovascular condition in which fat deposits and become oxidized along the inner lining of the artery walls. This silent yet deadly build up progressively thickens, hardens and eventually blocks the arteries leading to sudden and severe circulatory complications including vascular ischemia, stroke or heart attack. Cardiovascular and circulatory deaths related to atherosclerosis accounts for 29% of all deaths globally; the primary cause of death in EU (42%), Eastern Europe (48%), UK (39%), North America (49%), China (34%), South America (31%); Middle East (31%) and India (29%) – World Health Report, 2010.
The cardiovascular and circulatory benefits of natural astaxanthin are evident among Japanese who are the uppermost consumers of food containing astaxanthin (AX) in the world and have the lowest incidences of heart diseases amongst developed countries. As the French paradox of cardiovascular health is connected to “sipping red-wine” and Italians longevity to “olive oil dressed” salads, Japanese cardiovascular resilience can be associated with consumption of “astaxanthin-soaked” salmon. In fact, a growing number of scientific evidence points to a robust link between natural astaxanthin and cardiovascular health – 30 cardiovascular specific research publications including 10 clinical studies. Research suggests that oral supplementation of astaxanthin may reduce the risks of cardiovascular diseases by reducing hypertension while enhancing blood rheology, capillary circulation and vascular resilience.
For every 1 mg/dl increase in good cholesterol HDL, the risk of cardiovascular diseases drops by 3%. In fact, baby boomers with low-HDL (> 40mg/dL) increase their chances of experiencing coronary events by 50%. Recent studies suggest that individuals with low HDL cholesterol who also have high triglycerides levels are 11 times more likely to develop cardiovascular diseases. Achieving a significant increase of HDL is notoriously hard because it requires drastic lifestyle changes, so often ending with modest results or sudden relapses.
Recent research suggests that astaxanthin supplementation can support lifestyle changers by synergizing HDL increasing effect with decreased level of serum triglycerides. Two recent studies demonstrated that astaxanthin consumption can steadily increase HDL cholesterol in both healthy and less healthy individuals -both as preventive and therapeutic use. Yoshida et al., (2009) conducted the first ever randomized, placebo-controlled human study to evaluate astaxanthin effect on dyslipidemia and metabolic syndrome. Sixty-one hyper-triglyceride subjects between 42-47 years old (BMI 24 mg/kg), received 0 (placebo), 6 mg, 12mg, 18mg of astaxanthin daily for 12 weeks. While the placebo group did not change their existing condition, the astaxanthin groups increased their HDL cholesterol by 11%, 15% and 7% respectively and decreased their serum triglycerides level by 17%, 25% and 24% respectively (figure 1).
In a recent clinical study, 73 subjects between 20-60 years of age who received 4mg of natural astaxanthin per day for 4 weeks had their serum triglycerides level decreased by 25 %(Satoh et al., 2009). In another study conducted in Japan, 15 healthy adults increased their HDL by 6% after ingesting 9mg/daily of astaxanthin for 8 weeks (Matsumaya et al., 2010). In 2007, Hussein et al., has shown that astaxanthin reduced the size of fat cells in rats, which lead to a lower risk of cardiovascular complications and chronic inflammation (figure 2).
High levels of triglycerides and low levels of HDL also increase the likelihood of fat-oxidation in vessels and formation of “wounds” in the inner lining of artery walls (endothelium) leading to chronic inflammation and oxidative stress; this situation causes degradation, narrowing and thickening of arteries. Three recent clinical studies have robustly pointed to astaxanthin ability to reduce fat peroxidation in blood plasma. In a randomized-double-blind placebo study, 33 overweight subjects received 5mg or 20mg astaxanthin daily for 3 weeks. Their lipid peroxidation markers plasma MDA Level (mmol) and plasma ISP (ng/mL) decreased by 30% and 60% in average (Choi et al., 2011).
In another randomized double blind placebo controlled study, 30 subjects between 50 and 69 years of age received 0 (placebo), 6 or 12mg astaxanthin daily for 12 weeks (Nakagawa et al., 2011). The amount of oxidized red blood cells (PLOOH um0l/ml) decreased by 17% and 24% respectively(figure 3).
In 2007, Karppi et al., conducted a randomized double blind conducted placebo controlled study with 40 non-smoking subjects between 19-33 years of age who received 0 (placebo) or 8mg of astaxanthin daily for 12 weeks. Their lipid peroxidation markers -plasma-15-hydroxy fatty acidsdecreased by 60% and plasma-12-hydroxy fatty acids by 36%. In 2000, Iwamoto et al., has also shown that astaxanthin inhibited LDL oxidation in human subjects. Professor Aoi from Kyoto Prefectural University, has shown that astaxanthin limits exercise-induced cardiac oxidation damage in mice.
Low antioxidant activity in the blood correlates with high incidences of stroke, neurological impairment in stroke patients and cardiovascular diseases. Therefore, it is crucial to monitor the biomarkers of antioxidant capacity in the blood when assessing the efficacy of an active ingredient. In a randomized double blind study, 33 overweight subjects received 5mg or 20mg astaxanthin daily for 3 weeks. Their plasma Superoxide Dismutase Level (SOD) (U/mL) and Plasma Total Antioxidant Capacity (TAC) Level (mmol) increased 45% and 19% respectively. (Choi et al., 2011) (figure 4).
Other studies have produced similar results using different assessment methods. In an open label clinical study, 35 postmenopausal women were treated with astaxanthin daily dose of 12 mg for 8 weeks (Yonei et al., 2009). Astaxanthin supplementation increased biological antioxidant potential in the blood plasma by 5% in 8 weeks. In addition, Camera et al., suggested that astaxanthin protects and synergize with our endogenous antioxidant systems (superoxide dismutase, catalase and glutathione) from early degradation when subjected to oxidative stress (Camera et al., 2008).
In the presence of oxidized cells in the endothelial lesions, macrophages white blood cells infiltrate in affected areas to clear away pathogens and dead cells. Yet, in the attempt to clean up the oxidized areas, macrophages may get overweighed with excessive lipoproteins and unable to leave the artery walls. This peculiar but common situation triggers a cascade of chronic inflammatory responses and pro-oxidant activities that degraded the structural integrity of the vessels. Therefore, up-regulated activity of oxidized LDL via macrophage induced inflammation is central to the initiation and progression of atherosclerosis. They are closely associated with plaque development, aggravation and ruptures.
A recent study shows that astaxanthin decreased macrophage occupied lesion areas and therefore inflammation in the vessels of rabbits by 40% compared to control group (figure 5). Furthermore, rabbits that ingested 4mg astaxanthin everyday for 24 weeks decreased programmed cell death (apoptosis) by 42% and cell death (necrosis) by 17% in the aorta (Li et al., 2004).
In-vitro study provides further evidences that astaxanthin (5-10uM) decreases macrophages related activation (SR-A and CD36) by 48% and 58% respectively (Kishimoto et al., 2009). A recent animal studies show that astaxanthin could ameliorate endothelial dysfunction by significantly improving the level of substances important for the regulation of vascular integrity. In more details, treatment with astaxanthin for 42 days decreased serum oxidized LDL cholesterol, aortic MDA levels, attenuated endothelium-dependent vasodilatory to acetylcholine, up-regulate eNOS expression and decreased LDL cholesterol receptor expression (figure 6).
Animal studies have also shown that astaxanthin ameliorated structural changes in the blood vessels – reduction in wall thickness by 47% and improved vascular tone by 36% in spontaneously hypertensive rats (Hussein et al., 2006). Such structural changes was observed in the reduction of the number of branched elastin bands and improved vessel wall to lumen thickness ratio.
In another study, 24 weeks supplementation of natural astaxanthin reduced levels of MMP3 expression in the aorta of rabbits – a crucial factor that lead to a degradation of elastin and collagen structures which determines the mechanical properties of connective tissues in the vessels (figure 7). In the experiment, astaxanthin enhanced plaque stability leading to a significant reduction of plaque ruptures (Li et al., 2004).
Good circulation, quality of blood and resilient vessels are the key features required to fight development and progression of atherosclerosis. Blood rich in antioxidants bring nutrients and oxygen to organs while removing waste through a smooth vascular resilience and capillary flow.
Recent human studies suggest that 6mg daily of astaxanthin can enhance blood flow by 10% in terms of capillary transit time -how fast the blood runs through the vessels (Miyawaki et al., 2008). Another complementary study showed that astaxanthin decreased lower limb vascular resistance by 17% – the degree to which the blood vessels impede the flow of blood (Iwabayashi et al., 2009).(figure 8) High resistance causes an increase in blood pressure, which increases the workload of the heart. In 2005, Nagaki et al., conducted another randomized double-blind study in which 36 subjects who received oral astaxanthin, 6mg/day for 4 weeks experienced a 4% improvement in capillary blood flow (Nagaki et al., 2005).
A series of human studies suggest that astaxanthin decreases blood pressure by improving blood flow and vascular tone. In a recent clinical study, 73 subjects, between 20-60 years of age, who received 4mg of astaxanthin for day for 4 weeks showed a significant decrease in systolic blood pressure (Satoh et al., 2009). In another study, 15 healthy subjects, between 27-50 of age, who received 9mg/day of astaxanthin for 12 weeks had their diastolic blood pressure decreased significantly (Matsuyama et al., 2010).
A series of animal studies have largely replicated the effects of astaxanthin found in human studies (e.g. Ruiz et al., 2010; Preuss, 2011).
Clinical studies suggests that oral supplementation of natural astaxanthin (4mg-12mg) may reduce the risk cardiovascular complications by enhancing blood rheology, lipid-metabolism, capillary circulation, vascular resilience and the endogenous antioxidant defense. Other clinical studies have also shown that astaxanthin reduce lipid-peroxidation, LDL cholesterol, blood pressure and DNA damage. Mechanism of action includes inhibition of macrophage-induced inflammation in the endothelium, oxidative stress-induced apoptosis and MPP-induced-structural degradation of the vessels. Furthermore, recent studies have also outlined that astaxanthin ameliorates nitric oxide dependent vessels dilation and reduce sensitivity to the angiotensin.
CCRES special thanks to
Mr. Mitsunori Nishida,
President of Corporate Fuji Chemical Industry Co., Ltd.
Croatian Center of Renewable Energy Sources (CCRES)
An algal production facility located at the CCRES Research Farm will be operational by June. This is the first facility at Croatia that can produce large amounts of algal biomass.
The facility is a 800 square-foot greenhouse that will accommodate two raceway pond systems, four large flat panel photobioreactors and one custom-made revolving attachment-based photobioreactor. The total production capacity will be 100-200 dried kilograms of algae biomass per year.
CCRES Researchers will use the various production systems to quickly grow algal biomass for various research purposes including the production of renewable fuels, food or animal feed. “This greenhouse algal production system will be a test bed for different researchers to try out their algal production capability at a large scale,” said Zeljko Serdar, President of CCRES ALGAE TEAM.
“The raceway pond systems are each 20 feet in length and both systems can hold approximately 1,000 liters of algae culture medium. Raceway pond systems are the most common method for large-scale algae cultivation. At first glance, the four flat panel photobioreactors appear to be large tanks,” said Ilam Shuhani, Chairman of the CCRES Supervisory Board and professor-in-charge of the greenhouse.
Biodiesel from microalgae has a comparable quality as rapeseed methyl ester and meets the standard EN 14214. At biodiesel production about 12% glycerin is produced as a by-product. This glycerin is a valuable resource for the production of algae in closed ponds, the heterotrophic processes. Thus, the entire algae oil can be used as fuel.
High-protein fish food
Replacement for existing fish meal production
Algae have nutrients of many young fishes available
The fishing industry recorded an annual growth of over 10% and, according to experts, will beat the global beef consumption in 2015.
The Technology developed by CCRES offers the opportunity to deliver part of the needed proteins for fish farming on the resulting algal biomass.
The big growth opportunities are:
Fitness and Sports Nutrition
The market volume in the protein sector is continously growing and at the rate of US $ 10.5B in 2010 and according to experts, will steadily increase to approx. $25B until 2030.
Algae are emerging to be one of the most promising long-term, sustainable sources of biomass and oils for fuel, food, feed, and other co-products. What makes them so attractive are the large number and wide variety of benefits associated with how and where they grow.
Nearly all these benefits stem from the fact that these plants have evolved over billions of years to produce and store energy in the form of oil, and they do this more efficiently than any other known natural or engineered process.
Here are 10 reasons why algae are a promising new source of fuel and other products:
1) Algae Grow Fast
Algae can double their numbers every few hours, can be harvested daily, and have the potential to produce a volume of biomass and biofuel many times greater than that of our most productive crops.
2) Algae Can Have High Biofuel Yields
Algae store energy in the form of oils and carbohydrates, which, combined with their high productivity, means they can produce from 2,000 to as many as 5,000 gallons of biofuels per acre per year.
3) Algae Consume CO2
Like any other plant, algae, when grown using sunlight, consume (or absorb) carbon dioxide (CO2) as they grow, releasing oxygen (O2) for the rest of us to breathe. For high productivity, algae require more CO2, which can be supplied by emissions sources such as power plants, ethanol facilities, and other sources.
4) Algae Do Not Compete With Agriculture
Algae cultivation uses both land that in many cases is unsuitable for traditional agriculture, as well as water sources that are not useable for other crops, such as sea-, brackish- and wastewater. As such, algae-based fuels complement biofuels made from traditional agricultural processes.
5) Microalgal Biomass Can Be Used for Fuel, Feed and Food
Microalgae can be cultivated to have a high protein and oil content, for example, which can be used to produce either biofuels or animal feeds, or both. In addition, microalgal biomass, which is rich in micronutrients, is already used for dietary supplements to advance human health.
6) Macroalgae Can Be Grown in the Sea
Macroalgae (seaweeds) are grown in the sea, or even on land with seawater, and their sugars can be converted into biofuels and chemicals.
7) Algae Can Purify Wastewaters
Algae thrive in nutrient-rich waters like municipal waste waters (sewage), animal wastes and some industrial effluents, at the same time purifying these wastes while producing a biomass suitable for biofuels production.
8) Algal Biomass Can Be Used as an Energy Source
After oil extraction, the remaining algal biomass can be dried and “pelletized” and used as fuel that is burned in industrial boilers and other power generation sources.
9) Algae Can Be Used to Produce Many Useful Products
Algae can be cultivated to produce a variety of products for large to small markets: plastics, chemical feedstocks, lubricants, fertilizers, and even cosmetics. See other products algae is used for here.
10) The Algae Industry is a Job Creation Engine
Algae can grow in a wide variety of climates in a multitude of production methods, from ponds to photobioreactors to fermenters, and thus will create a wide variety of jobs throughout the United States, from research to engineering, from construction to farming, from marketing to financial services.
CCRES ALGAE TEAM